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SAB4200759

Sigma-Aldrich

Anti-Human IgG3 antibody, Mouse monoclonal

clone HP-6050, purified from hybridoma cell culture

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Synonym(s):
Anti-Immunoglobulin G3

biological source

mouse

Quality Level

200

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

HP-6050, monoclonal

form

buffered aqueous solution

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 1-2.5 μg/test using Human IgG3 myeloma
indirect ELISA: 0.15-0.3 μg/mL using 1 μg/ml Human IgG3 myeloma for coating

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Related Categories

General description

Human IgG consist of four subclasses (1-4) that can be recognized by antigenic differences in their heavy chains. They constitute approximately 65, 30, 5 and 4% of the total IgG, respectively. The abundance of the different IgG subclasses in the bloodstream varies with age, IgG1 and IgG3 reach normal adult levels by 5-7 years of age while IgG2 and IgG4 levels rise more slowly, reaching adult levels at about 10 years of age.

Immunogen

Human IgG3 myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads

Application

Anti-Human IgG3 antibody, mouse monoclonal has been used in indirect ELISA and immunoprecipitation.

Biochem/physiol Actions

IgG1 and IgG3 are the only subclasses capable of adherence to mononuclear phagocytes and are recognized readily by the Fc receptors on various reticulo-endothelial . IgG3 deficiency has been associated with a history of recurrent infectious, leading to chronic lung disease.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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R Jefferis et al.
Immunology letters, 10(3-4), 223-252 (1985-01-01)
Seventy-four monoclonal antibodies (McAb) of putative specificity for human IgG (11), the IgG sub-classes (59) or Gm allotypes (4) have been evaluated for reactivity and specificity in eight laboratories employing different assay techniques or protocols. For the IgG, IgG3, IgG4
Multiple sclerosis plasma IgG aggregates induce complement-dependent neuronal apoptosis.
Zhou, et al.
Cell Death & Disease, 14, 254-254 (2023)
Gestur Vidarsson et al.
Frontiers in immunology, 5, 520-520 (2014-11-05)
Of the five immunoglobulin isotypes, immunoglobulin G (IgG) is most abundant in human serum. The four subclasses, IgG1, IgG2, IgG3, and IgG4, which are highly conserved, differ in their constant region, particularly in their hinges and upper CH2 domains. These

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