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Merck
CN

SAB4200762

Anti-Opsin antibody, Mouse monoclonal

clone RET-P1, purified from hybridoma cell culture

Synonym(s):

Anti-Opsin 2, Anti-Rhodopsin

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203

Key Documents

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Product Name

Anti-Opsin antibody, Mouse monoclonal, clone RET-P1, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

RET-P1, monoclonal

form

buffered aqueous solution

mol wt

~39 kDa

species reactivity

avian, bovine, fish, mouse, rat, human, amphibian, turtle, rabbit

concentration

~1 mg/mL

technique(s)

ELISA: suitable
immunoblotting: 2-4 μg/mL using human retinoblastoma Y79 cell line extract.
immunofluorescence: suitable
immunohistochemistry: 5-10 μg/mL using rat eye frozen sections.
radioimmunoassay: suitable

isotype

IgG1

UniProt accession no.

P08100

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

rat ... Rho(24717)

Application

Anti-Opsin antibody has been used in
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry
  • enzyme linked immunosorbent assay (ELISA)
  • radioimmuno assay (RIA)

Biochem/physiol Actions

Opsin is an essential molecules for mediating the ability of animals to detect and use light for diverse biological functions. Mutations in the opsin (rhodopsin) gene are linked to retinitis pigmentosa (RP), a disease characterized by retinal degeneration resulting in reduced peripheral vision and night blindness.

General description

Anti-Opsin antibody, Mouse monoclonal (rhodopsin) (mouse IgG1 isotype) is derived from the RET-P1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/C mice immunized with rat retinal membranes. Opsin is also called as rhodopsin protein, members of the G-protein coupled receptor (GPCR) superfamily. Opsin comprises >95% of the rod outer segments (ROS) intrinsic membrane and consists of a protein moiety - an opsin - and a non-protein moiety - the chromophore retinal.

Other Notes

rat retinal membranes

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
057M4814V

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Find documentation for the products that you have recently purchased in the Document Library.

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Retinitis pigmentosa
Hamel C.
Orphanet Journal of Rare Diseases, 1(1), 40-40 (2006)
The opsins
Terakita A.
Genome Biology, 6(3), 213-213 (2005)

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