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SAB4200784

Anti-Cellular Fibronectin antibody, Mouse monoclonal

Name: Anti-Cellular Fibronectin antibody, Mouse monoclonal
Brand: SIGMA

clone FN-3E2, hybridoma cell culture supernatant

Synonym(s):

Anti-CIG, Anti-Cold-insoluble globulin, Anti-FN

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

FN-3E2, monoclonal

Application:

IF, IHC

Citations:

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Properties

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

culture supernatant

antibody product type

primary antibodies

clone

FN-3E2, monoclonal

form

buffered aqueous solution

mol wt

~220-260 kDa

species reactivity

chicken, rat, human, mouse

technique(s)

immunoblotting: suitable, immunofluorescence: 1:2,000-1:4,000 using human foreskin fibroblast Hs68 cells, immunohistochemistry: suitable

isotype

IgM

UniProt accession no.

P02751

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FN1(2335)

Description

General description

Anti-Cellular Fibronectin antibody, Mouse monoclonal (mouse IgM isotype) is derived from the FN-3E2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with the antigens released in culture from a breast cancer cell line. Fibronectin (FN), also known as cold-insoluble globulin (CIG), is an extracellular matrix multi-domain glycoprotein composed of two nearly identical disulphide bound polypeptides. Fibronectin 1 is a glycoprotein, that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35. Cellular fibronectin is produced by various cell types, including fibroblasts, endothelial cells, chondrocytes, synovial cells, and myocytes.

Immunogen

Antigens released in culture from a breast cancer cell line

Application

Anti-Cellular Fibronectin antibody, Mouse monoclonal has been used in:

immunoblotting
immunofluorescence
immunohistochemistry

Anti-cellular Fibronectin antibody, mouse monocloneal has been used in various immunochemical techniques including immunoblotting (220-260 kDa), immunofluorescence, and immunohistochemistry.

Biochem/physiol Actions

Fibronectin participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, haemostasis and thrombosis. It is a ubiquitous and essential component of the extracellular matrix (ECM) and plays a vital role in tissue repair mechanism.

Physical form

The product is supplied as a culture supernatant solution containing 15 mM sodium azide as a preservative. The product contains fetal calf serum.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

低风险生物材料

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number

0000458289

0000378308

0000338446