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Merck
CN

SAB4501457

Anti-HSP27 antibody produced in rabbit

affinity isolated antibody

Synonym(s):

28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Growth-related 25 kDa protein, HSP 27, Heat shock 27 kDa protein

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About This Item

MDL number:
MFCD00282279
UNSPSC Code:
12352203
NACRES:
NA.41

Key Documents

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biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 22 kDa

species reactivity

human, rat, mouse

concentration

~1 mg/mL

technique(s)

ELISA: 1:10000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

NCBI accession no.

P04792

UniProt accession no.

P04792

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HSPB1(3315)

General description

Anti-HSP27 Antibody detects endogenous levels of total HSP27 protein.

Immunogen

The antiserum was produced against synthesized peptide derived from human HSP27.

Immunogen Range: 48-97

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
217112

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Ye Gu et al.
iScience, 27(5), 109272-109272 (2024-05-06)
For a long time, myeloid-derived suppressor cells (MDSCs) dilated in circulation system of colorectal cancer (CRC) patients have been puzzling clinicians. Various evidence shows that MDSCs constitute the bulk of immunosuppression in CRC, which is related to tumor growth, adhesion
Monica Celi et al.
Methods in molecular biology (Clifton, N.J.), 2756, 343-350 (2024-03-01)
Heat shock proteins (HSPs) in all animals studied to date constitute potential indicators of stress, under various environmental conditions. The goal of this chapter is to show, for the first time, the suitability of the approach based on evaluation of
Masashi Fukuoka et al.
PloS one, 9(7), e103130-e103130 (2014-07-30)
Heat shock, sudden change in temperature, triggers various responses in cells for protecting the cells from such a severe circumstance. Here we investigated gene silencing mediated by endogenous microRNAs (miRNAs) in mammalian cells exposed to a mild hyperthermia, by means

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