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Safety Information

SAB4504169

Sigma-Aldrich

Anti-phospho-DNA-PK (pSer2056) antibody produced in rabbit

affinity isolated antibody

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Synonym(s):
Anti-DNA-PKC, Anti-DNA-PKcs, Anti-DNAPK, Anti-DNAPKc, Anti-DNPK1, Anti-HYRC, Anti-HYRC1, Anti-IMD26, Anti-XRCC7

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 469 kDa

species reactivity

human, mouse, rat

concentration

~1 mg/mL

technique(s)

ELISA: 1:10000
immunohistochemistry: 1:50-1:100

NCBI accession no.

P78527

UniProt accession no.

P78527

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer2056)

Gene Information

human ... PRKDC(5591)

General description

Protein kinase, DNA-activated, catalytic polypeptide (PRKDC), also known as DNAPK, is encoded by the gene mapped to human chromosome 8q11. PRKDC is characterized by a large catalytic polypeptide and DNA-binding component comprised of the Ku70 and Ku80 heterodimer, that targets DNA-PKc, to DNA.

Immunogen

The antiserum was produced against synthesized peptide derived from human DNA-PK around the phosphorylation site of Ser2056.

Immunogen Range: 2022-2071

Application

Anti-phospho-DNA-PK (pSer2056) antibody produced in rabbit has been used in immunofluorescence analysis.

Biochem/physiol Actions

Protein kinase, DNA-activated, catalytic polypeptide (PRKDC), also known as DNAPK, plays an essential role in the colorectal cancer (CRC) cell proliferation/survival. Therefore, it can be used as a potential prognostic biomarker and a therapeutic target for CRC. Decreased expression of PRKDC influences apoptosis partially by inhibiting AKT activation. Overexpression of activated DNAPK elevates the expression of rearranged during transfection (RET), which is a proto-oncogene involved in the development of medullary thyroid cancer (MTC). DNAPK plays a crucial role in non-homology end joining (NHEJ) pathway. Downregulation of DNAPK enhances anticancer drug induced DNA damage repair in osteosarcoma cell line MG63.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Information

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Shangfeng Sun et al.
Gene, 584(1), 90-96 (2016-03-20)
Oncogene and non-oncogene addictions describe the phenomenon that tumor cells become reliant on certain genes for maintenance of malignancy. Reversal of these mutations profoundly affects tumor growth and survival, providing a fundamental rationale for development of targeted cancer therapy. However
Xin Li et al.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 36(12), 9365-9372 (2015-06-26)
Many chemotherapy drugs exert anticancer effects through causing DNA damage, such as DNA topoisomerase inhibitor and platinum-containing drugs. DNA damage repair is an important mechanism of drug resistance which is responsible for metastasis and recurrence after chemotherapy. DNA-dependent protein kinase
J D Sipley et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(16), 7515-7519 (1995-08-01)
The DNA-activated serine/threonine protein kinase (DNA-PK) is composed of a large (approximately 460 kDa) catalytic polypeptide (DNA-PKcs) and Ku, a heterodimeric DNA-binding component (p70/p80) that targets DNA-PKcs to DNA. A 41-kbp segment of the DNA-PKcs gene was isolated, and a
Weiwei Li et al.
Mutation research, 779, 112-123 (2015-07-24)
Although studies have shown that cadmium (Cd) interfered with DNA damage repair (DDR), whether Cd could affect non-homologous end joining (NHEJ) repair remains elusive. To further understand the effect of Cd on DDR, we used X-ray irradiation of Hela cells
Luca Mattiello et al.
International journal of molecular sciences, 22(3) (2021-02-13)
Cancer treatments can often adversely affect the quality of life of young women. One of the most relevant negative impacts is the loss of fertility. Cyclophosphamide is one of the most detrimental chemotherapeutic drugs for the ovary. Cyclophosphamide may induce
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