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SAB4700581

Sigma-Aldrich

Monoclonal Anti-CD11b, low endotoxin antibody produced in rat

clone M1/70, purified immunoglobulin, buffered aqueous solution

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Synonym(s):
Anti-Itgam, Anti-Mac-1 α
NACRES:
NA.41

biological source

rat

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M1/70, monoclonal

form

buffered aqueous solution

species reactivity

human, nonhuman primates, mouse

concentration

1 mg/mL

technique(s)

flow cytometry: suitable

isotype

IgG2b

NCBI accession no.

NM_001082960.1

UniProt accession no.

P11215

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

mouse ... Itgam(16409)

Related Categories

General description

Cluster of differentiation 11b (CD11b), also known as integrin subunit α M (ITGAM), is encoded by the gene mapped to human chromosome 16p11.2. The encoded protein belongs to the integrin α chain family. CD11b is expressed by a variety of myeloid cell types, such as dendritic cells, macrophages, monocytes and neutrophils.
The rat monoclonal antibody M1/70 detects CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.

Immunogen

B10 mouse spleen cells enriched for T cells

Application

The reagent is designed for Flow Cytometry analysis. Suggested working dilution for Flow Cytometry is 1 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Biochem/physiol Actions

Cluster of differentiation 11b (CD11b) acts as a receptor for complement component C3 degradation product, iC3b. CD11b interacts with β2 chain (ITGB2) to form a leukocyte integrin (MAC-1 or complement receptor 3 (CR3)), which in turn plays a crucial role in adherence of neutrophils and monocytes to stimulated endothelium. Mutation in the gene increases the risk of susceptibility to systemic lupus erythematosus (SLE) and acute inflammatory demyelinating polyradiculoneuropathy (AIDP).

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Solution in azide free phosphate buffered saline, pH 7.4; 0.2 um filter sterilized. Endotoxin level is less than 10 EU/mg of the protein, as determined by the LAL test.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Genome-wide association scan in women with systemic lupus erythematosus identifies susceptibility variants in ITGAM, PXK, KIAA1542 and other loci
Harley JB
Nature Genetics, 40, 204-210 (2008)
J Tan et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 20(20), 7587-7594 (2000-10-12)
Reactive microglia have been suggested to play a role in the Alzheimer's disease (AD) process, and previous studies have shown that expression of CD45, a membrane-bound protein-tyrosine phosphatase (PTP), is elevated in microglia in AD brain compared with controls. To
The pathogenic relevance of αM-integrin in Guillain-Barre syndrome.
Dong C
Acta Neuropathologica, 132, 739-752 (2016)
A nonsynonymous functional variant in integrin-alpha(M) (encoded by ITGAM) is associated with systemic lupus erythematosus.
Nath SK
Nature Genetics, 40, 152-154 (2008)
Association of Systemic Lupus Erythematosus with C8orf13?BLK and ITGAM?ITGAX
Hom G
The New England Journal of Medicine, 358, 900-909 (2008)
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