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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Conjugate:
phycoerythrin (R-PE) conjugate
Clone:
DCS-6, monoclonal
Application:
FACS
Citations:
-
conjugate
phycoerythrin (R-PE) conjugate
antibody product type
primary antibodies
clone
DCS-6, monoclonal
form
buffered aqueous solution
species reactivity
( Human), ( Mouse), rat, ( Non-human primates)
concentration
0.1 mg/ml
technique(s)
flow cytometry: suitable
isotype
IgG2a
UniProt accession no.
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CCND1(595)
General description
Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation, cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.
Immunogen
recombinant human cyclin D1 (amino acids 1-295)
Biochem/physiol Actions
The mouse monoclonal antibody DCS-6 recognizes cyclin D1, an ubiquitously expressed 33 kDa intracellular protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.
Physical form
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Preparation Note
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Other Notes
The antibody solution should be gently mixed before use.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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