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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Conjugate:
unconjugated
Clone:
M5/114, monoclonal
Application:
FACS, FUNC, IP, WB
Citations:
-
conjugate
unconjugated
antibody product type
primary antibodies
clone
M5/114, monoclonal
form
buffered aqueous solution
species reactivity
mouse
concentration
1 mg/ml
technique(s)
FUNC: suitable, flow cytometry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
Quality Level
isotype
IgG2b
storage temp.
2-8°C
target post-translational modification
unmodified
General description
MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
Immunogen
Activated C57BL/6 mouse spleen cells
Biochem/physiol Actions
The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared extracellular determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
Physical form
Phosphate buffered saline (PBS), pH 7.4
Preparation Note
Purified by protein-G affinity chromatography.
Store at 2-8°C. Do not freeze.
Other Notes
The antibody solution should be gently mixed before use.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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