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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Conjugate:
PE-Cy7 conjugate
Clone:
MEM-181, monoclonal
Application:
flow cytometry
Species reactivity:
human
Citations:
Technique(s):
flow cytometry: suitable
Uniprot accession no.:
conjugate
PE-Cy7 conjugate
antibody product type
primary antibodies
clone
MEM-181, monoclonal
form
buffered aqueous solution
species reactivity
human
technique(s)
flow cytometry: suitable
isotype
IgG1
UniProt accession no.
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... IL2RA(3559)
Biochem/physiol Actions
The antibody MEM-181 reacts with an extracellular epitope of CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
General description
CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.
Immunogen
PHA-activated peripheral blood leucocytes
Other Notes
The antibody solution should be gently mixed before use.
Physical form
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Preparation Note
Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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