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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Conjugate:
biotin conjugate
Clone:
MEM-259, monoclonal
Application:
FACS, ICC, IHC, IP
Citations:
-
conjugate
biotin conjugate
Quality Segment
antibody product type
primary antibodies
clone
MEM-259, monoclonal
form
buffered aqueous solution
species reactivity
human
concentration
1 mg/ml
technique(s)
flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable
isotype
IgG1
UniProt accession no.
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... CD63(967)
General description
CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.
Immunogen
HPB-ALL T cell line
Biochem/physiol Actions
The antibody MEM-259 reacts with an extracellular/luminal epitope of CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.
Physical form
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Preparation Note
Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
Store at 2-8°C. Do not freeze.
Other Notes
The antibody solution should be gently mixed before use.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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