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Merck
CN

SAB4702062

Monoclonal Anti-HLA-E purified produced in mouse

Synonym(s):

HLA class I histocompatibility antigen, MHC class I antigen E, alpha chain E

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
Conjugate:
unconjugated
Clone:
MEM-E/02, monoclonal
Application:
immunohistochemistry
western blot
Species reactivity:
( Non-human primates), human
Citations:
Technique(s):
immunohistochemistry: suitable
western blot: suitable
Uniprot accession no.:
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conjugate

unconjugated

antibody product type

primary antibodies

clone

MEM-E/02, monoclonal

form

buffered aqueous solution

species reactivity

( Non-human primates), human

concentration

1 mg/ml

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

storage temp.

2-8°C

target post-translational modification

unmodified, unmodified

Quality Level

Gene Information

human ... HLA-E(3133)

Biochem/physiol Actions

The antibody MEM-E/02 specifically reacts with an extracellular epitope on denaturated heavy chain of human HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of all human cell types.

General description

HLA-E (human leukocyte antigen E) is a non-classical MHC I antigen, which is important for dialogue with NK cells and their regulation through interaction with CD94/NKG2 receptor. Like other MHC I molecules, transmembrane HLA-E molecule (45 kDa) associates with beta2 microglobulin. Unlike HLA-G, expression of HLA-E molecules is not so restricted, but it has been detected at least at mRNA level in virtually all cells and tissues examined. In peripheral blood, HLA-E protein is expressed at least in all mononuclear cells, but in different quantity (B cells and monocytes more than T cells and NK cells).

Immunogen

Recombinant human HLA-E denaturated heavy chain.

Other Notes

The antibody solution should be gently mixed before use.

Physical form

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preparation Note

Purified by protein-A affinity chromatography.
Store at 2-8°C. Do not freeze.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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