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Merck
CN

SAB5702016

Anti-eIF4A1 Antibody, clone 7D5K2, Rabbit Monoclonal

Synonym(s):

DDX2A, EIF-4A, EIF4A, eIF-4A-I, eIF4A-I

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About This Item

NACRES:
NA.46
UNSPSC Code:
12352203

Key Documents

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biological source

rabbit

conjugate

unconjugated

material

colorless

clone

7D5K2, monoclonal

form

liquid

mol wt

46 kDa

species reactivity

human, rat, rat

concentration

0.256 mg/mL

technique(s)

immunofluorescence: 1:50 - 1:200
immunohistochemistry: 1:50 - 1:200
immunoprecipitation (IP): 1:50 - 1:200
western blot: 1:500 - 1:2000

color

colorless

isotype

IgG

immunogen sequence

MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKA

UniProt accession no.

P60842

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... EIF4A1(1973)

Application

WB, IHC, IF/ICC, IP

General description

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5′-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF4A1 (P60842).
PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.

Storage Class

10 - Combustible liquids

wgk

WGK 1

Regulatory Information

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