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Merck
CN

SAB5702253

Sigma-Aldrich

Anti-Prdm9 Antibody, clone 1H9Z1, Rabbit Monoclonal

Synonym(s):

Rc, re, Dsbc, Rcr1, Dsbc1, Meise, repro7, Meisetz, PRDM9-B, BC012016, G1-419-29

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

Key Documents

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biological source

rabbit

Quality Level

100

conjugate

unconjugated

material

colorless

clone

1H9Z1, monoclonal

form

liquid

mol wt

110 kDa

species reactivity

rat, human, rat

concentration

1 mg/mL

technique(s)

immunohistochemistry: 1:50 - 1:200
western blot: 1:500 - 1:1000

color

colorless

isotype

IgG

immunogen sequence

DSEDSDEEWTPKQQVSPPWVPFRVKHSKQQKESSRMPFSGESNVKEGSGIENLLNTSGSEHVQKPVSSLEEGNTSGQHSGKKLKLRKKNVEVKMYRLRERKGLAYEEVSEPQDDDYLYCEKCQNFFIDSCPNHGPPLFVKDSMVDRGHPNHSVLSLPPGLRISPSGIPEAGLGVWNEASDLPVGLHFGPYEGQITEDEEAANSGYSWLITKGRNCYEYVDGQDESQANWMRYVNCARDDEEQNLVAFQYHRKIFYRTCRVIRPGCELLVWYGDEYGQELGIKWG

UniProt accession no.

Q96EQ9

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Prdm9(213389)

General description

Histone methyltransferase that sequentially mono-, di-, and tri-methylates both ′Lys-4′ (H3K4 and ′Lys-36′ (H3K36 of histone H3 to produce respectively trimethylated ′Lys-4′ (H3K4me3 and trimethylated ′Lys-36′ (H3K36me3 histone H3 and plays a key role in meiotic prophase by determining hotspot localization thereby promoting meiotic recombination. Also can methylate all four core histones with H3 being the best substrate and the most highly modified. Is also able, on one hand, to mono and di-methylate H4K20 and on other hand to trimethylate H3K9 with the di-methylated H3K9 as the best substrate. During meiotic prophase, binds specific DNA sequences through its zinc finger domains thereby determining hotspot localization where it promotes local H3K4me3 and H3K36me3 enrichment on the same nucleosomes through its histone methyltransferase activity. Thereby promotes double-stranded breaks (DSB formation, at this subset of PRDM9-binding sites, that initiates meiotic recombination for the proper meiotic progression.

Immunogen

PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Recombinant fusion protein containing a sequence corresponding to amino acids 87-370 of mouse Prdm9 (NP_659058.3).

Application

WB, IHC

Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H317,H411

Hazard Classifications

Aquatic Chronic 2 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Regulatory Information

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