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Merck
CN

SAB5702374

Sigma-Aldrich

Anti-RPA70/RPA1 Antibody, clone 5R7S3, Rabbit Monoclonal

Synonym(s):

HSSB, MST075, REPA1, RF-A, RP-A, RPA70

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

Key Documents

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biological source

rabbit

Quality Level

100

conjugate

unconjugated

material

colorless

clone

5R7S3, monoclonal

form

liquid

mol wt

70 kDa

species reactivity

human, rat

concentration

0.55 mg/mL

technique(s)

ChIP: 1:50 - 1:200
immunoprecipitation (IP): 1:50 - 1:200
western blot: 1:500 - 1:2000

color

colorless

isotype

IgG

immunogen sequence

MVGQLSEGAIAAIMQKGDTNIKPILQVINIRPITTGNSPPRYRLLMSDGLNTLSSFMLATQLNPLVEEEQLSSNCVCQIHRFIVNTLKDGRRVVILMELE

UniProt accession no.

P27694

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RPA1(6117)

General description

As part of the heterotrimeric replication protein A complex (RPA/RP-A, binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation.

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPA70/RPA1 (P27694).
PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.

Application

WB, IP, ChIP

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Information

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