Skip to Content
Merck
CN

SAB6010029

Sigma-Aldrich

Anti-Histone H3.3 antibody, clone 4H2, Rabbit Monoclonal

recombinant, expressed in HEK293F cells

Synonym(s):

Histone H3.3, H3F3A, H3.3A, H3F3, PP781, AND, H3F3B, H3.3B

Sign Into View Organizational & Contract Pricing

Select a Size

About This Item

UNSPSC Code:
12352203

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

recombinant

expressed in HEK 293 F cells

Quality Level

100

conjugate

unconjugated

antibody product type

primary antibodies

clone

4H2, recombinant monoclonal

form

liquid

mol wt

150 kDa

purified by

affinity chromatography

species reactivity

human

concentration

2mg/ml

technique(s)

ELISA: 2000:10000
flow cytometry: 1:50-1:200
immunofluorescence: 1:50-1:200
immunohistochemistry: 1:50-1:200

isotype

IgG

UniProt accession no.

P84243

shipped in

wet ice

storage temp.

-10 to -25°C

target post-translational modification

unmodified

General description

Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Immunogen

A synthesized peptide derived from human Histone H3.3

Physical form

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Preparation Note

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Other Notes

70-100aa

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

新产品
This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service