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Merck
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SAB6010046

Anti-phospho-EIF2S1 (S51) antibody, clone 1C6, Rabbit Monoclonal

recombinant, expressed in HEK293F cells

Synonym(s):

Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, EIF2S1, EIF2A

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
Conjugate:
unconjugated
Clone:
1C6, recombinant monoclonal
Application:
ELISA, FACS, IF, IHC
Citations:
-

Key Documents

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recombinant

expressed in HEK 293 F cells

conjugate

unconjugated

antibody product type

primary antibodies

clone

1C6, recombinant monoclonal

form

liquid

mol wt

150 kDa

purified by

affinity chromatography

species reactivity

human

concentration

1.5mg/ml

technique(s)

ELISA: 2000:10000, flow cytometry: 1:50-1:200, immunofluorescence: 1:50-1:200, immunohistochemistry: 1:50-1:200

isotype

IgG

UniProt accession no.

P05198

shipped in

wet ice

storage temp.

-10 to -25°C

Quality Level

100

target post-translational modification

phosphorylation (pSer51)

General description

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Immunogen

A synthesized peptide derived from human Phospho-EIF2S1 (S51)

Physical form

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Preparation Note

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Other Notes

28-68aa

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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