SAE0005
Glucose-6-phosphate Isomerase human
recombinant, expressed in E. coli
Synonym(s):
GPI, PGI
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recombinant
expressed in E. coli
Quality Segment
assay
≥95% (SDS-PAGE)
specific activity
≥200 units/mg protein
storage temp.
−20°C
General description
C-terminal histidine-tagged
Biochem/physiol Actions
Glucose-6-phosphate isomerase (GPI) is a homodimeric enzyme ubiquitously present in most organisms. GPI catalyzes the interconversion between glucose-6-phosphate and fructose-6-hosphate, the second step of the glycolytic pathway. In mammals, Glucose-6-phosphate isomerase also acts as an autocrine motility factor (AMF), a neuroleukin, and a maturation factor. GPI deficiency is the second most common erythroenzymopathy of glycolytic enzymes after pyruvate kinase deficiency. Inherited deficiency of the enzymatic activity of GPI causes hereditary nonspherocytic hemolytic anemia (HNSHA) in humans, a severe deficiency that can be associated with hydrops fetalis (a-thalassaemia), immediate neonatal death, and neurological impairment.
Physical form
Supplied as a solution in 50 mM Tris-HCl pH 7.5, and 50% glycerol
Other Notes
One unit will convert 1.0 mmole of D -fructose 6-phosphate to D -glucose 6-phosphate per inute at pH 7.4 at 25 °C.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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