SAE0023
Alanine Racemase from Bacillus stearothermophilus
buffered aqueous solution, ≥10 IU/mg solid
Synonym(s):
Alanine Racemase from Bacillus stearothermophilus
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥10 IU/mg solid
Biological source:
Bacillus sp. (B. stearothermophilus)
biological source
Bacillus sp. (B. stearothermophilus)
form
buffered aqueous solution
specific activity
≥10 IU/mg solid
mol wt
Mr 78 kDa (2 subunits 39 kDa each)
storage temp.
−20°C
Quality Level
Application
Alanine racemase is used to convert L-alanine into D-alanine. Alanine racemase, from Sigma, has been used to isomerize L-[U-14C]alanine to a racemic mixture of L/D- [14C]alanine †.
Biochem/physiol Actions
Alanine racemase is involved in alanine, aspartate and D-alanine metabolism. 3-Fluoro-D-alanine and D-Cycloserine are known to inhibit alanine racemase.† Alanine racemase monomer is composed of two domains, an eight-stranded α/β barrel at the N-terminus and a C-terminal domain. The N-terminus includes residues 1-240, whereas the C-terminal comprises of the β-strand (residues 241-388). One molecule of pyridoxalphosphate (PLP) is present as the cofactor in each subunit.†
Physical form
Aqueous solution containing 10 mM potassium phosphate
Other Notes
One unit will convert 1.0 μmole of D-alanine to L-alanine per minute at pH 10.5 at 30 °C in a coupled assay system with L-alanine dehydrogenase.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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