SAE0045
HRV-3C Protease.
N-Terminal His tagged recombinant protein, aqueous solution, 0.8-1.2 mg/mL
Synonym(s):
Human Rhinovirus 3C Protease, Levlfqgp site protease, PreScission Protease
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About This Item
NACRES:
NA.54
UNSPSC Code:
12352204
Specific activity:
≥5000 U/mg
Assay:
≥90% (SDS-PAGE)
Biological source:
human (human Rhinovirus Type 14 )
Concentration:
0.8-1.2 mg/mL
biological source
human (
Quality Segment
assay
≥90% (SDS-PAGE)
form
aqueous solution
specific activity
≥5000 U/mg
mol wt
21 kDa
concentration
0.8-1.2 mg/mL
technique(s)
protein purification: suitable
suitability
suitable for protein modification
application(s)
life science and biopharma
shipped in
dry ice
storage temp.
−20°C
General description
HRV-3C protease from human rhinovirus type 14 is a protease that specifically cleaves within an eight-residue recognition sequence.
Proteolytic cleavage occurs between the Gln and Gly residues.
HRV-3C protease is a useful tool to cleave recombinant proteins that are expressed as a fusion protein with this sequence, between the carrier domain and the protein of interest.
This recombinant version contains a six-histidine tag and can be easily removed by IMAC chromatography.
Proteolytic cleavage occurs between the Gln and Gly residues.
HRV-3C protease is a useful tool to cleave recombinant proteins that are expressed as a fusion protein with this sequence, between the carrier domain and the protein of interest.
This recombinant version contains a six-histidine tag and can be easily removed by IMAC chromatography.
Application
HRV-3C Protease has been used to remove the GST tag from the DsCystatin protein.
Physical form
Supplied in a solution containing 50 mM Tris HCl (pH 7.5), 0.15 M NaCl, 1 mM TCEP and 50% (V/V) glycerol.
Other Notes
One unit of HRV-3C Protease is defined as the amount of enzyme needed to digest 1nmole of the substrate peptide per hour (H-Glu-Ala-Leu-Phe-Gln-pNA) at 0 °C, in a reaction buffer consisting of 25 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM EDTA, and 1 mM DTT.
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Storage Class
10 - Combustible liquids
wgk
WGK 2
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