Skip to Content
Merck
CN

SAE0069

Cholera Toxin B subunit

≥95% (SDS-PAGE), lyophilized powder, enterotoxin

Synonym(s):

CTB, CTxB, Cholera enterotoxin B chain, Cholera enterotoxin gamma chain, Cholera enterotoxin subunit B, Choleragenoid, ToxB

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Cholera Toxin B subunit, recombinant, expressed in HEK 293 cells, subunit A free

recombinant

expressed in HEK 293 cells

assay

≥95% (SDS-PAGE)

form

lyophilized powder

specific activity

(Binds to GM1 ganglioside. The binding EC50 is lower than 1 ug/ml)

mol wt

pentamer observed mol wt ~70 kDa by SDS-PAGE (non-reducing)
calculated mol wt 13 kDa
observed mol wt ~13 & 20 kDa by SDS-PAGE (reducing)

solubility

H2O: soluble 11 mg/mL

UniProt accession no.

P01556

storage temp.

−20°C

Quality Level

200

Related Categories

Analysis Note

Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with ≤1 μg of Cholera toxin B subunit per mL.

Application

Cholera Toxin B subunit has been used:
  • for macrophage stimulation and i.p. injection in a study to determine the endotoxin sensitivity of Caspase-4.
  • in transganglionic and retrograde tract-tracing method combined with dual-immunofluorescence histochemistry of adult rat Vmes neuron cells.

General description

The advantage of this product over the native CTXB is that it has no traces of Vibrio cholera, and no contamination of the highly toxic Cholera toxin A subunit. This product is expressed in human HEK 293 cells as a C-terminally his-tagged protein with a calculated molecular mass of 13 kDa (amino acids Thr22-Asn124).
The protein is recombinant, and therefore has no contamination of the highly toxic Cholera toxin A subunit.
Under non-reducing conditions, it migrates as pentamer of ~70 kDa, consistent with the pentamerization of CTXB.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.

Preparation Note

When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 1X PBS

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品
This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000233296
0000233296
0000209509
0000138318
0000209509

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Si-Yuan Song et al.
Frontiers in neuroanatomy, 14, 600555-600555 (2020-12-18)
This study aimed to investigate the direct monosynaptic projections from cortical functional regions to the cerebrospinal fluid (CSF)-contacting nucleus for understanding the functions of the CSF-contacting nucleus. The Sprague-Dawley rats received cholera toxin B subunit (CB) injections into the CSF-contacting

Related Content

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service