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SAE0069

Cholera Toxin B subunit

Name: Cholera Toxin B subunit
Brand: SIGMA

≥95% (SDS-PAGE), lyophilized powder, enterotoxin

Synonym(s):

CTB, CTxB, Cholera enterotoxin B chain, Cholera enterotoxin gamma chain, Cholera enterotoxin subunit B, Choleragenoid, ToxB

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About This Item

UNSPSC Code:

12352200

NACRES:

NA.32

Assay:

≥95% (SDS-PAGE)

Form:

lyophilized powder

Quality level:

200

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Product Name

Cholera Toxin B subunit, recombinant, expressed in HEK 293 cells, subunit A free

recombinant

expressed in HEK 293 cells

Quality Level

200

assay

≥95% (SDS-PAGE)

form

lyophilized powder

specific activity

(Binds to GM1 ganglioside. The binding EC50 is lower than 1 ug/ml)

mol wt

pentamer observed mol wt ~70 kDa by SDS-PAGE (non-reducing), calculated mol wt 13 kDa, observed mol wt ~13 & 20 kDa by SDS-PAGE (reducing)

solubility

H₂O: soluble 11 mg/mL

UniProt accession no.

P01556

storage temp.

−20°C

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Bioactive Small Molecules

General description

The advantage of this product over the native CTXB is that it has no traces of Vibrio cholera, and no contamination of the highly toxic Cholera toxin A subunit. This product is expressed in human HEK 293 cells as a C-terminally his-tagged protein with a calculated molecular mass of 13 kDa (amino acids Thr22-Asn124).

The protein is recombinant, and therefore has no contamination of the highly toxic Cholera toxin A subunit.

Under non-reducing conditions, it migrates as pentamer of ~70 kDa, consistent with the pentamerization of CTXB.

Application

Cholera Toxin B subunit has been used:

for macrophage stimulation and i.p. injection in a study to determine the endotoxin sensitivity of Caspase-4.
in transganglionic and retrograde tract-tracing method combined with dual-immunofluorescence histochemistry of adult rat Vmes neuron cells.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.

Preparation Note

When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 1X PBS

Analysis Note

Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with ≤1 μg of Cholera toxin B subunit per mL.

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品

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Certificates of Analysis (COA)

Lot/Batch Number

0000519338

0000233296

0000209509

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The CSF-Contacting Nucleus Receives Anatomical Inputs From the Cerebral Cortex: A Combination of Retrograde Tracing and 3D Reconstruction Study in Rat.

Si-Yuan Song et al.

Frontiers in neuroanatomy, 14, 600555-600555 (2020-12-18)

This study aimed to investigate the direct monosynaptic projections from cortical functional regions to the cerebrospinal fluid (CSF)-contacting nucleus for understanding the functions of the CSF-contacting nucleus. The Sprague-Dawley rats received cholera toxin B subunit (CB) injections into the CSF-contacting

Global Trade Item Number

SKU	GTIN
SAE0069-500UG	04061833265376

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