SAE0104
BmrA (Bacilus Subtilis Multidrug Resistance Abc Transporter Atp-Binding Protein)
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About This Item
NACRES:
NA.83
UNSPSC Code:
12352202
Form:
aqueous solution
Assay:
≥90% (SDS-PAGE)
Recombinant:
expressed in E. coli
Mol wt:
64.5 kDa
recombinant
expressed in E. coli
description
C-Terminal six-histidine tagged
assay
≥90% (SDS-PAGE)
form
aqueous solution
mol wt
64.5 kDa
concentration
0.3 mg/mL
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
General description
MSSSHHHHHHMPTKKQKSKSKLKPFFALVRRTNPSYGKLAFALALSVVTTLVSLLIPLLTKQLVDGFSMSNLSGTQIGLIALVFFVQAGLSAYATYALNYNGQKIISGLRELLWKKLIKLPVSYFDTNASGETVSRVTNDTMVVKELITTHISGFITGIISVIGSLTILFIMNWKLTLLVLVVVPLAALILVPIGRKMFSISRETQDETARFTGLLNQILPEIRLVKASNAEDVEYGRGKMGISSLFKLGVREAKVQSLVGPLISLVLMAALVAVIGYGGMQVSSGELTAGALVAFILYLFQIIMPMGQITTFFTQLQKSIGATERMIEILAEEEEDTVTGKQIENAHLPIQLDRVSFGYKPDQLILKEVSAVIEAGKVTAIVGPSGGGKTTLFKLLERFYSPTAGTIRLGDEPVDTYSLESWREHIGYVSQESPLMSGTIRENICYGLERDVTDAEIEKAAEMAYALNFIKELPNQFDTEVGERGIMLSGGQRQRIAIARALLRNPSILMLDEATSSLDSQSEKSVQQALEVLMEGRTTIVIAHRLSTVVDADQLLFVEKGEITGRGTHHELMASHGLYRDFAEQQLKMNADLENKAG
Biochem/physiol Actions
BmrA is an efflux protein and ABC transporter that acts as an ATP-dependent drug efflux pump.
Preparation Note
Formulated in 50mM Tris-Cl pH 8.0, 100mM NaCl, 0.01% DDM, 10% glycerol.
Thaw on ice. Upon first thaw, briefly spin tube containing protein to recover full content of the tube. Aliquot into single use aliquots. Store remaining undiluted protein in aliquots at -80°C. Note: avoid freeze/thaw cycles.
wgk
WGK 1
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Emmanuelle Steinfels et al.
Biochemistry, 43(23), 7491-7502 (2004-06-09)
The involvement of transporters in multidrug resistance of bacteria is an increasingly challenging problem, and most of the pumps identified so far use the protonmotive gradient as the energy source. A new member of the ATP-binding cassette (ABC) family, known
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