SAE0212
Mucin Probe, StcE
Biotin and FLAG® tagged, powder
Synonym(s):
Catalytically inactive Mucin specific protease StcE
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About This Item
NACRES:
NA.77
UNSPSC Code:
12352202
Form:
powder
General description
Mucin Probe, StcE is a proteolytically inactive mutant (E447D) form of Mucinase StcE that maintains ability to tightly bind mucin proteins. The recombinant protein carries an N-terminal Hig-Tag® and 3XFLAG™ tag at the C-term end of the protein which is also enzymatically biotinylated. While there are many applications, this product is designed to probe mucin dense tissues. It will non-covalently, and selectively bind to Mucin-domain glycoproteins with dense O-linked glycosylation. Secondary reporting tools can then be used to bind the probe using its FLAG® tag or Biotin conjugate, as described in the Product Information Sheet.
Legal Information
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk
WGK 2
Regulatory Information
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Keira E Mahoney et al.
Current protocols, 4(7), e1100-e1100 (2024-07-10)
Mucin-domain glycoproteins are characterized by their high density of glycosylated serine and threonine residues, which complicates their analysis by mass spectrometry. The dense glycosylation renders the protein backbone inaccessible to workhorse proteases like trypsin, the vast heterogeneity of glycosylation often
Articles
Pretreatment with Mucinase StcE increases glycopeptide identification from mucin samples, enhancing sample preparation efficiency for glycopeptide analysis.
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