Hygromycin B

Name: Hygromycin B
Brand: SIGMA

Min. 95% (HPLC ELSD), from Streptomyces hygroscopicus

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):

C₂₀H₃₇N₃O₁₃

CAS Number:

31282-04-9

Molecular Weight:

527.52

UNSPSC Code:

51281624

NACRES:

NA.76

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Product Name

Hygromycin B, Min. 95% (HPLC ELSD), from Streptomyces hygroscopicus

biological source

Streptomyces hygroscopicus

Quality Level

200

form

solid

antibiotic activity spectrum

Gram-negative bacteria, Gram-positive bacteria, fungi, viruses

mode of action

protein synthesis | interferes

storage temp.

2-8°C

SMILES string

N([C@@H]1[C@H]([C@@H]([C@H]([C@@H](C1)N)O)O[C@@H]2O[C@@H]([C@@H]([C@@H]3O[C@@]4(O[C@@H]32)O[C@@H]([C@@H]([C@@H]([C@H]4O)O)O)[C@H](N)CO)O)CO)O)C

InChI

1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1

InChI key

GRRNUXAQVGOGFE-XKIAHZFYSA-N

Description

General description

Chemical structure: aminoglycoside

Application

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes.

Biochem/physiol Actions

Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.