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SBR00049

FITC Labeled D-Alanine

Suitable for bacterial labeling

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About This Item

Empirical Formula (Hill Notation):
C24H19N3O7S
Molecular Weight:
493.49
NACRES:
NA.24
UNSPSC Code:
12352209
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Quality Segment

form

solid

storage temp.

−20°C

General description

Bacterial Peptidoglycan is a polymer consisting of sugars and amino acids, a mesh-like rigid layer that forms the cell wall. D-Amino Acids (D-AAs) are metabolically incorporated onto the bacterial cell wall by D,D-transpeptidase and/or L,D-transpeptidase. It was recently discovered that those transpeptidases catalyze the metabolic incorporation of exogenous D-AAs with almost no restriction of the side-chain identity. Modification of a D-AA with molecular fluorophores provides fluorescent d-amino acids that can efficiently label in situ peptidoglycan in diverse bacterial species.
Fluorescein Labeled D-Alanine is a fluorescent derivative of D-alanine that is obtained by a synthetic conjugation of 3-amino-D-alanine to a fluorophore. Fluorescein Labeled D-Alanine has high biocompatibility and suitability for labeling peptidoglycans in live bacteria

Application

Fluorescent labeled D-AAs can be used for many applications including:
  • Bacterial cell wall morphology
  • Bacterial cell wall formation or remodeling activity
  • Bacterial viability/activity
  • Identify bacterial activity on surfaces or in substances

Analysis Note

  • Fluorescent microscopy application: Fluorescein Labeled D-Alanine has excitation/emission wavelength range at 495 nm/515 nm.
  • The recommended working concentration in fluorescent microscopy imaging application is between 250 μM-500 μM in DMSO.
  • Aliquots of the DMSO solution can be stored at -20°C, protected from light for at least one month.

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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