SCP0007
ADAM substrate
≥95% (HPLC), lyophilized
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About This Item
Empirical Formula (Hill Notation):
C64H85N13O18S2
Molecular Weight:
1388.57
UNSPSC Code:
12352202
NACRES:
NA.32
Product Name
ADAM substrate, ≥95% (HPLC)
assay
≥95% (HPLC)
form
lyophilized
composition
Peptide Content, ≥70%
storage condition
protect from light
storage temp.
−20°C
Application
ADAM substrate containing the fluorophore quencher Dabcy is used in FRET applications for detection of metalloprotease activity. DABCYL is a popular acceptor for developing FRET-based protease substrates. DABCYL dyes are often paired with EDANS in FRET-based fluorescent probes.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Marcia L Moss et al.
Analytical biochemistry, 366(2), 144-148 (2007-06-06)
In this paper we describe novel fluorescent substrates for the human ADAM family members ADAM17, ADAM10, ADAM8, and ADAM12 that have good specificity constants and are useful for high-throughput screening of inhibitors. The fluorescence resonance energy transfer substrates contain a
Characterization of Mca-Lys-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2, a fluorogenic substrate with increased specificity constants for collagenases and tumor necrosis factor converting enzyme.
Neumann U, Kubota H, Frei K, et al.
Analytical Biochemistry, 328, 166-173 (328)
Bernhard F Becker et al.
Biological chemistry, 383(11), 1821-1826 (2003-01-18)
Tumor necrosis factor-alpha (TNFalpha) is presumably shed from cell membranes by TNFalpha-cleaving enzyme (TACE). The peptides SPLAQAVRSSSR and Dabcyl-LAQAVRSSSR-Edans, each encompassing the cleavage sequence of pro-TNFalpha recognized by TACE, were applied to intact umbilical vein endothelium (HUVEC), peripheral blood leukocytes
Marcia L Moss et al.
Combinatorial chemistry & high throughput screening, 13(4), 358-365 (2009-12-18)
Fluorescence resonance energy transfer substrates were designed and tested as substrates for ADAM9. The donor/quencher pair used were 5-carboxy fluorescein (Fam) and 4-(4-dimethyl-aminophenylazo)benzoyl (Dabcyl) since they have been well studied sensitive fluorescent probes. The peptides based on precursor TNF-alpha, Dabcyl-Ser-Pro-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Lys(Fam)-
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