Seppro^™ Rubisco

RuBisCO (Ribulose-1,5-bisphosphate carboxylase/oxygenase) is the most abundant protein in plants, and it may be the most abundant protein on Earth. RuBisCO is an enzyme that is used in the Calvin cycle to catalyze the first major step of carbon fixation, a process by which the atoms of atmospheric carbon dioxide are made available to organisms in the form of energy-rich molecules such as sucrose. RuBisCO, while being the key enzyme in photosynthetic carbon assimilation in green leaves, is the main obstacle in plant proteomics. It constitutes about 40% of the total protein mass in green leaves, thus interfering with proteomics studies such as LC-MS/MS and 2D-gel electrophoresis. To meet the needs for specifically and effectively separating RuBisCO from other plant proteins, Sigma developed a novel immunoaffinity matrix based on the IgY antibodies cross-linked to microbeads, which specifically removes RuBisCO protein from plant extract.

Seppro^™ Rubisco, spin columns (capacity: ∼0.2 mg of Rubisco) may be used in plant proteomics research to effect IgY-mediated immune affinity removal of RuBisCO (Ribulose-1,5-bisphosphate carboxylase/oxygenase) from plant tissue extracts via liquid chromatography.

Seppro is a trademark of Merck KGaA, Darmstadt, Germany