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SHC002

Sigma-Aldrich

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA

Targets no known mammalian genes

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Synonym(s):
MISSION® Control Vectors
MDL number:
NACRES:
NA.51

product line

MISSION®

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

shipped in

dry ice

storage temp.

−20°C

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Application

MISSION® pLKO.1-puro non-mammalian shRNA control plasmid DNA has been used:
  • to transduce RLE-6TN cells
  • for the validation of Oct4-green fluorescent protein (GFP) mouse embryonic fibroblasts (MEFs)
  • as a negative control for RNA knockdown

MISSION®pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA has been used:
  • for the knockdown of transmembrane prostate androgen-induced RNA (TMEPAI)
  • in the infection mixture for lentivirus preparation
  • for generating the puromycin-resistant ST14A (rat striatal neuronal cell line) cell populations
  • to induce Ago2 (argonaute-2) down-regulation

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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The metastasis suppressor NME1 inhibits melanoma cell motility via direct transcriptional induction of the integrin beta-3 gene
Leonard MK, et al.
Experimental Cell Research, 374(1), 85-93 (2019)
TRIM28 is an epigenetic barrier to induced pluripotent stem cell reprogramming
Miles DC, et al.
Stem Cells, 35(1), 147-157 (2017)
MicroRNA-101 regulates amyloid precursor protein expression in hippocampal neurons
Vilardo E, et al.
The Journal of Biological Chemistry, 285(24), 18344-18351 (2010)
Platelet-independent adhesion of calcium-loaded erythrocytes to von Willebrand factor.
Smeets MW
PLoS ONE, 12.3 (2017)
JNK1 Deficient Insulin-Producing Cells Are Protected against Interleukin-1?-Induced Apoptosis Associated with Abrogated Myc Expression.
Prause M
Journal of Diabetes Research (2016)

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