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SHC202

Sigma-Aldrich

MISSION® TRC2 pLKO.5-puro Non-Mammalian shRNA Control Plasmid DNA

Targets no known mammalian genes

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Synonym(s):
MISSION® Control Vectors
MDL number:
MFCD07785395
NACRES:
NA.51

Quality Level

200

product line

MISSION®

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

shipped in

dry ice

storage temp.

−20°C

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General description

This shRNA non-mammalian control was designed using our Turbo GFP sequence and may cause some knockdown of tGFP. For maximum knockdown of tGFP, please refer to SHC004, SHC004V, SHC004H, SHC204, or SHC204V.

The MISSION® TRC2 Control Vector pLKO-puro Non-Target shRNA is a lentivirus plasmid vector. This vector is in the TRC2 pLKO-puro plasmid backbone, which contains the WPRE.

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (SHP001). The TRC2 Non-Target shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Application

Small interfering RNAs (siRNAs) expressed from short hairpin RNAs (shRNAs) are a powerful way to mediate gene specific RNA interference (RNAi) in mammalian cells. The MISSION® product line is based on a viral vector-based RNAi library against annotated mouse and human genes. shRNAs that generate siRNAs intracellularly are expressed from amphotropic lentivirus viral particles, allowing screening in a wide range of mammalian cell lines. In these cell lines, MISSION® shRNA clones permit rapid, cost efficient loss-of-function and genetic interaction screens. The vector contains an shRNA insert that does not target human or mouse genes, making it useful as a negative control in experiments using the MISSION® TRC2 shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

recommended

Product No.
Description
Pricing

SHC201

MISSION® TRC2 pLKO.5-puro Empty Vector Control Plasmid DNA, Contains no shRNA insert

SHC203

MISSION® TRC2 pLKO.5-puro-CMV-TurboGFP™ Positive Control Plasmid DNA, Green fluorescent protein marker to monitor transduction efficiency

SHC204

MISSION® TRC2 pLKO.5-puro TurboGFP™ shRNA Control Plasmid DNA, shRNA sequence targeting tGFP

SHC201V

MISSION® TRC2 pLKO.5-puro Empty Vector Control Transduction Particles puro, Contains no shRNA insert

SHC202V

MISSION® TRC2 pLKO.5-puro Non-Mammalian shRNA Control Transduction Particles, Targets no known mammalian genes

SHC203V

MISSION® TRC2 pLKO.5-puro-CMV-TurboGFP™ Positive Control Transduction Particles, Green fluorescent protein marker to monitor transduction efficiency

SHC204V

MISSION® TRC2 pLKO.5-puro TurboGFP™ shRNA Control Transduction Particles, shRNA sequence targeting tGFP

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Regulatory Information

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Adaptor protein-3: A key player in RBL-2H3 mast cell mediator release.
da Silva EZ
PLoS ONE, 12(3), e0173462-e0173462 (2017)
Tianhe Huang et al.
Nature communications, 11(1), 2124-2124 (2020-05-03)
Penile squamous cell carcinoma (PSCC) accounts for over 95% of penile malignancies and causes significant mortality and morbidity in developing countries. Molecular mechanisms and therapies of PSCC are understudied, owing to scarcity of laboratory models. Herein, we describe a genetically
MYC Is a Crucial Mediator of TGF?-Induced Invasion in Basal Breast Cancer.
Cichon MA
Cancer Research, 76(12), 3520-3530 (2016)
Succination is Increased on Select Proteins in the Brainstem of the NADH dehydrogenase (ubiquinone) Fe-S protein 4 (Ndufs4) Knockout Mouse, a Model of Leigh Syndrome.
Piroli CG
Molecular and Cellular Proteomics, 15(2), 445-461 (2016)
Lou-Ella M M Alexander et al.
Molecular immunology, 91, 8-16 (2017-09-01)
B cell activation is dependent on a large increase in transcriptional output followed by focused expression on secreted immunoglobulin as the cell transitions to an antibody producing plasma cell. The rapid transcriptional induction is facilitated by the release of poised
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