SHP002
CRISPR & MISSION® Lentiviral Packaging Mix
Synonym(s):
Lentiviral Packaging Mix
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About This Item
concentration
100 ng/μL (in Tris-EDTA)
application(s)
CRISPR
shipped in
dry ice
storage temp.
−20°C
Quality Level
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Application
General description
Lentiviral particles are generated from three main components:
- The packaging vector, which contains the minimal set of lentiviral genes required to generate the virion structural proteins and packaging functions.
- The vesicular stomatitis virus G-protein envelope vector, which provides the heterologous envelope for pseudotyping and allows these lentiviral particles to efficiently deliver the transfer sequence of interest to a wide variety of cell types, including primary and non-dividing cells
- The lentiviral transfer vector, which contains the sequence of interest as well as the cis acting sequences necessary for packaging.
The Lentiviral Packaging Mix contains the first two components; it is designed to be co-transfected along with a compatible lentiviral transfer vector in order to create high-titer pseudo-typed lentiviral particles used for downstream transduction applications. This product is recommended for packaging Lenti CRISPR, Lenti ORF, and Lenti shRNA vectors.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Articles
Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.
Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.
获取处理慢病毒、优化实验设置、测定慢病毒颗粒滴度以及选择实用转导产品方面的贴士。
Protocols
Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.
FACS sorts cells based on light scattering and fluorescence for objective cell analysis.
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Instructions
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