SIALICQ
Sialic Acid Quantitation Kit
Sufficient for 25 reactions
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About This Item
NACRES:
NA.32
UNSPSC Code:
41116107
General description
The Sialic Acid Quantitation Kit provides a rapid and accurate determination of total N-acetylneuraminic acid (NANA; sialic acid). NANA is released from glycoconjugates using neuraminidase. The kit uses α(2→3,6,8,9) neuraminidase to cleave all NANA linkages, including α(2→8) and α(2→9) linkages, as well as branched NANA, for the most accurate determination of total sialic acid content.
Application
Contains all reagents to quantitate the amount of N-acetylneuraminic acid, either free, or in glycoproteins, cell surface glycoproteins, polysialic acids and capsular polysaccharides (consisting only of polysialic acid).
Preparation Note
Sufficient reagents are provided to perform 25 reactions, including control samples. Each reaction will measure 1-200 nmoles of NANA.
Kit Components Only
Product No.
Description
- N-Acetylneuraminic acid aldolase 25 μL
- N-Acetylneuraminic acid 200 μL
- α(2→3,6,8,9)-Neuraminidase 25 μL
- Fetuin, bovine .5 mg
- Lactic dehydrogenase 25 μL
- β-NADH disodium salt 3 vial(s)
- Tris-HCl, pH 7.5 1 mL
See All (8)
Storage Class
10 - Combustible liquids
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