SML3448
Propidium monoazide
≥98% (HPLC), Fluorescent DNA cross-linker, powder
Synonym(s):
3-Amino-8-azido-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridium dichloride, Propidium monoazide dichloride
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About This Item
Empirical Formula (Hill Notation):
C27H32N6·Cl2
CAS Number:
Molecular Weight:
511.49
UNSPSC Code:
12352125
NACRES:
NA.77
Product Name
Propidium monoazide, ≥98% (HPLC)
InChI key
NUMTTYQOTJKZSE-UHFFFAOYSA-M
SMILES string
[N-]=[N+]=NC1=CC2=C(C=C1)C3=C(C=C(C=C3)N)[N+](CCC[N+](CC)(C)CC)=C2C4=CC=CC=C4.[Cl-].[Cl-]
assay
≥98% (HPLC)
form
powder
color
red to black
solubility
DMSO: 2 mg/mL, clear
storage temp.
-10 to -25°C
Quality Level
Related Categories
Biochem/physiol Actions
Propidium derivative that allows light-induced crosslinkage to DNA for highly selective live-dead cells analysis by PCR or fluorescence microscopy.
Propidium monoazide (PMA) is a propidium derivative with an azide group that allows crosslinkage to DNA upon light exposure. PMA is only permeable to bacteria with compromised membrane integrity, making it highly selective for sick/dying/dead cells over healthy cells. PMA is typically added to cell samples (50 μM for 1-15 min in the dark) prior to cross-linking induction (650-W halogen light source), washing and DNA extraction. Due to its higher charge, PMA (+2) is less permeant to live cells and more selective than ethidium monoazide/EMA (+1) for dead cells. The stained cells can also be viewed and analyzed by fluorescence microscopy.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Whole microbial community viability is not quantitatively reflected by propidium monoazide sequencing approach
Microbiome, 9(1), 17-17 (2021)
Rapid profiling of drug-resistant bacteria using DNA-binding dyes and a nanopore-based DNA sequencer
Scientific Reports, 11(1), 3436-3436 (2021)
Detection and Potential Virulence of Viable but Non-Culturable (VBNC) Listeria monocytogenes: A Review
Microorganisms, 9(1), 194-194 (2021)
First Report on the Rapid Detection and Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) in Viable but Non-culturable (VBNC) Under Food Storage Conditions
Frontiers in Microbiology, 11, 615875-615875 (2021)
Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells
Journal of Microbiological Methods, 67(2), 310-320 (2006)
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