Skip to Content
Merck
CN

SML3688

Hypoxia probe 4

≥95% (HPLC)

Synonym(s):

5-Methoxy-1,2-dimethyl-3-(((3-oxo-9-(O-tolyl)-3H-xanthen-6-yl)oxy)methyl)-1H-indole-4,7-dione

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

Empirical Formula (Hill Notation):
C32H25NO6
Molecular Weight:
519.54
UNSPSC Code:
41116158
NACRES:
NA.77

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

InChI

1S/C32H25NO6/c1-17-7-5-6-8-21(17)29-22-11-9-19(34)13-26(22)39-27-14-20(10-12-23(27)29)38-16-24-18(2)33(3)31-25(35)15-28(37-4)32(36)30(24)31/h5-15H,16H2,1-4H3

InChI key

MQKQLIOGOATWPT-UHFFFAOYSA-N

SMILES string

CN1C(C(C=C2OC)=O)=C(C2=O)C(COC3=CC=C(C(C4=CC=CC=C4C)=C5C=C6)C(OC5=CC6=O)=C3)=C1C

assay

≥95% (HPLC)

form

powder

storage condition

protect from light

color

, Faint yellow to yellow-brown

solubility

DMSO: 2 mg/mL, clear

shipped in

wet ice

storage temp.

-10 to -25°C

Quality Level

100

Biochem/physiol Actions

Hypoxia probe 4 is a Tokyo green-based non-fluorescent hypoxia probe that undergoes cytochrome P450 reductase (PH51)-catalyzed reduction to yield the fluorescent reporter Tokyo green (ex 480 nm, em 515 nm) in conditions of 0.5% O2 and lower, allowing imaging hypoxia levels similar to those detected by EF5 without the use of antibodies. When employed together with resorufin-based hypoxia probe 2 (ex 545 nm, em 582 nm), the probe pair allows the simultaneous (two-color) imaging of different hypoxia levels in living cells.
Tokyo green-based hypoxia probe that allows fluorescent imaging of hypoxia levels in live cells similar to those detected by EF5 without the use of antibodies.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

新产品
This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000263183
0000263183

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Two Color Imaging of Different Hypoxia Levels in Cancer Cells
Journal of the American Chemical Society, 145(4), 2572-2583 (2023)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service