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Merck
CN

SML4235

MTSEA-Biotin-XX

≥98% (HPLC), powder, Thiol-reactive probe

Synonym(s):

MTSEA-xx-Biotin, Biotin-XX-MTSEA, S-(2-(6-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)hexanamido)ethyl) methanesulfonothioate

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About This Item

Empirical Formula (Hill Notation):
C25H45N5O6S3
CAS Number:
Molecular Weight:
607.85
UNSPSC Code:
12352200
Assay:
≥98% (HPLC)
Form:
powder
Quality level:
Storage condition:
desiccated
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Quality Level

assay

≥98% (HPLC)

form

powder

storage condition

desiccated

color

white to beige

solubility

DMSO: 2 mg/mL, clear (warmed)

shipped in

wet ice

storage temp.

-10 to -25°C

General description

MTSEA biotin-XX is a membrane-impermeant crosslinker consisting of biotin-coupled 2-aminoethyl-methanethiosulfonate (MTSEA).

Application

MTSEA-Biotin-XX may be used to covalently label cysteine residues in proteins or thiol-containing molecules with a biotin tag for streptavidin-based detection, purification, or accessibility studies and, for biotinylation of 4TU‐labeled RNAs in direct RNA sequencing.

Biochem/physiol Actions

MTSEA-Biotin-XX selectively and efficiently labels cell surface thiols, including cysteine residues and specific RNA modifications (s4U and dm6A), forming disulfide bonds with enhanced reactivity. The extended -XX- linker facilitates optimal biotin-streptavidin interaction, while the overall structure allows for high-yield, rapid reactions. Biotin-XX-MTSEA enables imaging, purification, and sequencing applications through its ability to tag target molecules via methanethiosulfonate (MTS) reactions.

Disclaimer

Hygroscopic, Store with desiccant

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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