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About This Item
Empirical Formula (Hill Notation):
C21H18F3N3O4 · xC2HF3O2
Molecular Weight:
433.38 (free base basis)
UNSPSC Code:
12352200
Assay:
≥98% (HPLC)
Form:
powder
Storage condition:
desiccated
Quality Segment
assay
≥98% (HPLC)
form
powder
storage condition
desiccated
color
white to beige
solubility
H2O: 2 mg/mL, clear
storage temp.
-10 to -25°C
Biochem/physiol Actions
H-Gly-Phe-AFC (GF-AFC) is a cell-permeable dipeptidyl peptidase 1 (DPP1; cathepsin C; cathepsin J) substrate commonly used for cell viability accessment, where the fluorophore AFC is cleaved off by intracellular DPP1 activity in live cells. Unlike ATP and MTT assays, GF-AFC assay requires no lysis at the end of the assay, so cells can be used afterwards for other live cell-based assays. Cells are typically incubated with GF-AFC at a final concentration of 80 µM (from a 100 mM stock in DMSO) for 0.5-1 h at 37°C, and the AFC fluorescence intensity can be monitored by a fluorescence plate reader or by a fluorescence microscope (Ex 380-400 nm and Em 505 nm).
Disclaimer
Hygroscopic, store with desiccant
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
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