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SML4301

Desthiobiotin-NHS

Name: Desthiobiotin-NHS
Brand: SIGMA

≥95% (HPLC), powder, amine-reactive NHS tag

Synonym(s):

2,5-Dioxopyrrolidin-1-yl 6-((4R,5S)-5-methyl-2-oxoimidazolidin-4-yl)hexanoate, Desthiobiotin N-hydroxysuccinimidyl ester, NHS-Desthiobiotin,N-Hydroxysuccinimido dethiobiotinate

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About This Item

Empirical Formula (Hill Notation):

C₁₄H₂₁N₃O₅

CAS Number:

80750-24-9

Molecular Weight:

311.33

Assay:

≥95% (HPLC)

Form:

powder

Quality level:

100

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Properties

Quality Level

100

assay

≥95% (HPLC)

form

powder

color

white to beige

solubility

DMSO: 2 mg/mL, clear

storage temp.

-10 to -25°C

Description

Application

Desthiobiotin-NHS may be used in protein modification, bioconjugation, and pull-down assays.

Biochem/physiol Actions

Desthiobiotin-NHS, an amine-reactive NHS tag, facilitates efficient and reversible protein enrichment. The desthiobiotin handle, a structural analog of biotin lacking a sulfur atom, exhibits a reduced binding affinity for avidin/streptavidin (µM KD) compared to biotin (pM KD), enabling gentle elution through competitive displacement with biotin or by weakening the interaction with mild alkali, thereby preserving protein integrity. This NHS tag readily conjugates to protein amines, enabling flexible, multi-cycle binding, washing, and elution. Note: the lower affinity of the desthiobiotin handle requires careful optimization to minimize non-specific interactions and potential loss during washes compared to biotin-streptavidin systems.

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number

0000505561

0000505562