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SNC10

mirPremier® microRNA Isolation Kit

1 sufficient for 10 preparations

Synonym(s):

microRNA Isolation Kit

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About This Item

NACRES:
NA.52
UNSPSC Code:
41105501
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Quality Level

200

usage

sufficient for 10 preparations

technique(s)

DNA extraction: suitable

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General description

Click here to view the kit protocol
Sigma′s mirPremier microRNA Isolation Kit provides a rapid and efficient method for purifying and enriching miRNAs and other small RNAs from diverse biological sources, including mammalian cell cultures, animal tissues, plant tissues, and microbial cultures, without using hazardous organic extractions. microRNAs (miRNAs) are a class of small RNA molecules, about 21 nucleotides (nt) in length, that regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. In addition, the kit also can be used for isolating total RNA if messenger RNA or other large RNAs are of interest.
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Application

mirPremier® microRNA Isolation Kit has been used to:
  • prepare small RNA
  • enrich small RNAfractions from the human astrocytes for small RNA sequencing and bioinformatics analysis
  • isolate small RNA from Vigna mungo tissues
  • extract miRNAs to detect the expression of miRNA-16

Features and Benefits

  • Developed to enhance the efficiency of isolating microRNA and other small RNA molecules directly from a wide range of biological samples.
  • Facilitates rapid and effective extraction and concentration of miRNA in 30 minutes for downstream applications.
  • Capable of extracting miRNA with high purity with no detectable large RNA.
  • Hazardous organic extractions are not involved.

Legal Information

mirPremier is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Only

Product No.
Description
  • Binding Solution 2
  • Lysis Solution

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
0000437271
0000437271
0000279482
0000279482
SLCN8852

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Extraction of Small RNA and qPCR Validation of miRNAs in Vigna mungo
Paul S, et al.
Bio-protocol, 5(5), e1417-e1417 (2015)
Xiaoqiong Tang et al.
Cancer management and research, 12, 2119-2125 (2020-04-14)
LncRNA mortal obligate RNA transcript (MORT) is downregulated in different types of cancer, indicating its involvement in cancer biology. In this study, MORT and miRNA-16 were both downregulated in plasma of mantle cell lymphoma (MCL) patients than that in the
Ying Zhang et al.
Genome biology, 18(1), 105-105 (2017-06-16)
Post-transcriptional regulation of gene expression can be achieved through the control of mRNA stability, cytoplasmic compartmentalization, 3' UTR length and translational efficacy. Spermiogenesis, a process through which haploid male germ cells differentiate into spermatozoa, represents an ideal model for studying
MicroRNAs control mRNA fate by compartmentalization based on 3? UTR length in male germ cells
Zhang Y, et al.
Genome Biology, 18(1), 105-105 (2017)
Jiayong Xie et al.
Regenerative therapy, 21, 87-95 (2022-07-06)
Diabetic nephropathy (DN) is a severe diabetic complication and podocyte damage is a hallmark of DN. The Nucleoporin 160 (NUP160) gene was demonstrated to regulate cell proliferation and apoptosis in mouse podocytes. This study explored the possible role and mechanisms
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mirPremier microRNA Isolation Kit (SNC10 SNC50)

取扱説明書

mirPremier microRNA Isolation Kit

microRNA, mirPremier microRNA Isolation Kit

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Global Trade Item Number

SKUGTIN
SNC10-1KT04061837108013

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