SRE0005
Proteinase K from Tritirachium album
Reagents designed and manufactured under current ISO 13485 certification.
Synonym(s):
Endopeptidase K
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
254-457-8
MDL number:
Biological source:
microbial
Concentration:
≥10 mg/mL protein, ≥800 unit/mL
biological source
microbial
grade
Molecular Biology
form
buffered aqueous glycerol solution
mol wt
28.93 kDa
storage condition
dry at room temperature
concentration
≥10 mg/mL protein, ≥800 unit/mL
technique(s)
DNA extraction: suitable, RNA extraction: suitable
impurities
≤500 pg/mg DNA (PicoGreen® assay)
color
almost colorless
pH
7.5
suitability
suitable for gel electrophoresis and DNA isolation procedures
Quality Level
application(s)
diagnostic assay manufacturing
foreign activity
DNAse, RNAse, exonuclease, endonuclease, and nickase, none detected
shipped in
wet ice
storage temp.
2-8°C
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General description
Proteinase K is a stable serine protease with broad substrate specificity. It degrades many proteins in the native state even in the presence of detergents. Proteinase K was isolated from a fungus able to grow on keratin and the enzyme can digest native keratin (hair), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity.
Application
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
Biochem/physiol Actions
Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.
Features and Benefits
Features:
- Qualified Raw Materials: Products that meet the stringent requirements of the Elevated Assurance Program, ensuring compliance with regulatory standards.
- Comprehensive Documentation: Includes essential documents such as certificates, impurity profiles, and declarations to support audits and risk assessments.
- M-Clarity™ Quality Attributes: Products are classified within the MQ300 quality segment or higher, demonstrating superior quality assurance. M-Clarity™ Program
- Confirmed Process Controls: Established processes that ensure consistency and reliability in manufacturing.
- Enhanced Change Notification: Timely updates on any changes to product specifications or processes, ensuring transparency and compliance.
- Freely Available Dossiers: Access to robust, ready-to-present product dossiers that include:
- Site quality assessment
- ISO 9001 certificate
- Detailed product specifications
- Relevant declarations/statements (e.g., animal origin content, BSE/TSE, nitrosamine)
- Regulatory Compliance: Helps you meet compliance standards effectively, reducing the risk of regulatory issues.
- Audit Readiness: Instills confidence during audits with comprehensive documentation and confirmed process controls, demonstrating your commitment to high-quality products.
- Operational Transparency: Supports supply chain transparency, allowing you to track and verify the quality of raw materials.
- Enhanced Support: Provides exceptional quality-related information and tailored assistance to meet your specific needs.
- Risk Mitigation: Facilitates thorough risk assessments with detailed documentation, helping you manage potential compliance challenges.
- Informed Decision-Making: Access to clear and comprehensive product information enables better decision-making and enhances operational efficiency.
Other Notes
One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Legal Information
M-CLARITY is a trademark of Merck KGaA, Darmstadt, Germany
PicoGreen is a registered trademark of Life Technologies
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Nikol Jankovska et al.
Biomedicines, 10(3) (2022-03-26)
Creutzfeldt-Jakob disease (CJD), the most common human prion disorder, may occur as "pure" neurodegeneration with isolated prion deposits in the brain tissue; however, comorbid cases with different concomitant neurodegenerative diseases have been reported. This retrospective study examined correlations of clinical
Jian Liu et al.
Applied and environmental microbiology, 79(18), 5593-5600 (2013-07-10)
Stenotrophomonas maltophilia is an important global opportunistic pathogen for which limited therapeutics are available because of the emergence of multidrug-resistant strains. A novel bacteriocin, maltocin P28, which is produced by S. maltophilia strain P28, may be the first identified phage
Brian J Conti et al.
Nature structural & molecular biology, 21(3), 228-235 (2014-02-25)
Eukaryotic secretory proteins cross the endoplasmic reticulum (ER) membrane through a protein-conducting channel contained within the ribosome-Sec61translocon complex (RTC). Using a zinc-finger sequence as a folding switch, we show that cotranslational folding of a secretory passenger inhibits translocation in canine
Jessica Varela Villarreal et al.
Journal of microbiological methods, 94(3), 161-169 (2013-07-03)
Molecular techniques, such as polymerase chain reaction (PCR) and quantitative PCR (qPCR), are very sensitive, but may detect total DNA present in a sample, including extracellular DNA (eDNA) and DNA coming from live and dead cells. DNase I is an
Eva Dvorakova et al.
BMC neurology, 13, 126-126 (2013-09-26)
The accumulation of the misfolded forms of cellular prion protein, i.e. prions (PrPSc), in the brain is one of the crucial characteristics of fatal neurodegenerative disorders, called transmissible spongiform encephalopathies (TSEs). Cellular prion protein is normally linked to the cell
Articles
Balancing Proteinase K cost with quality and technical support ensures optimal enzyme selection for diverse applications.
Guidelines on use of proteinase K, an enzyme commonly used to degrade proteins, and protect DNA and RNA from degradation in samples.
In blood DNA extraction, Proteinase K, an enzyme commonly used to degrade proteins, can help break down the cellular and nuclear membranes, releasing DNA from the cells that protect it from degradation and increase purity/yield making it more suitable for various molecular biology techniques.
Pro K aids in disrupting cell membranes for DNA release, crucial for downstream molecular biology techniques.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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