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Merck
CN

SRE0007

Mutanolysin from Streptomyces globisporus ATCC 21553

Suitable for manufacturing of diagnostic kits and reagents, ≥4000 units/mg protein (biuret)

Synonym(s):

Mutanolysin

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About This Item

CAS Number:
55466-22-3
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
MFCD00131635

Key Documents

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Product Name

Mutanolysin from Streptomyces globisporus ATCC 21553, Suitable for manufacturing of diagnostic kits and reagents, ≥4000 units/mg protein (biuret)

biological source

bacterial (Streptomyces globisporus)

form

lyophilized powder

specific activity

≥4000 units/mg protein (biuret)

mol wt

23 kDa

concentration

≥40% protein

suitability

suitable for cell lysis

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

Quality Level

400

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Application

Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.

Biochem/physiol Actions

Mutanolysin cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. It lyses Listeria and other gram positive bacteria such as Lactobacillus and Lactococcus.
Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.

General description

Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control

Other Notes

One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.

Physical form

Lyophilized powder containing Ficoll® and sodium succinate buffer salts

Legal Information

Ficoll is a registered trademark of Cytiva

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000452301
0000452300
0000452300
0000452301
0000445759

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