SRE0023
β-Glucuronidase from abalone
Purified, aqueous solution, β-glucuronidase 150,000-250,000 units/mL, β-glucuronidase ≥20,000,000 units/g protein
Quality Level
form
aqueous solution
β-glucuronidase activity
≥20,000,000 units/g protein
150,000-250,000 units/mL
secondary activity
≤7,500 units/mL sulfatase
storage temp.
2-8°C
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Related Categories
Application
Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine.
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine.
Biochem/physiol Actions
β-Glucuronidase (GUSB) is a lysosomal enzyme, that degrades glucuronate-containing glycosaminoglycans. β-glucuronidase, obtained from abalone has the ability to hydrolyze samples, that has high analyte concentrations. Absence of β-glucuronidase results in mucopolysaccharidosis type VII (MPSVII), which leads to lysosomal storage in the brain.
Physical form
Supplied as an aqueous solution containing L-proline, ammonium acetate and sodium azide
Other Notes
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).
Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
低风险生物材料
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Evaluation of abalone beta-glucuronidase substitution in current urine hydrolysis procedures
Malik W B, et al.
Journal of Analytical Toxicology, 38(3), 171-176 (2014)
Human beta-glucuronidase: structure, function, and application in enzyme replacement therapy
Naz H, et al.
Rejuvenation Research, 16(5), 352-363 (2013)
Articles
β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.
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