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Merck
CN

SRE0069

Phosphoglucomutase from rabbit muscle

Synonym(s):

α-D-Glucose-1,6-bisphosphatase, α-D-Glucose-1-phosphate phosphotransferase

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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
MDL number:
Specific activity:
≥100 units/mg protein
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form

lyophilized powder

Quality Segment

specific activity

≥100 units/mg protein

foreign activity

Lactic dehydroenase ≤0.5%, Phosphoglucose Isomerase ≤0.01%, pyruvate kinase ≤0.05%

shipped in

wet ice

storage temp.

−20°C

General description

Research area: CellSignalling. The phosphoglucomutase (PGM) molecule, a single polypeptide chain, is made up of four α/β domains. The domains form a compact heart-shaped structure with a large fissure between the two lobes, and the molecule comprises about 40 secondary structural components. The active enzymatic site of the molecule is located at the bottom of the crevice on the surface of domain I. Rabbit muscle phosphoglucomutase (RB-PGM) is a monomer with a unique four-domain architecture.

Application

Phosphoglucomutase from rabbit muscle may be used in dephosphorylation to investigate the hexose bisphosphate activation of phosphoglucomutase.

Biochem/physiol Actions

Phosphoglucomutase (PGM) mainly catalyzes the interconversion of glucose 1-phosphate and glucose 6-phosphate via a glucose 1,6-diphosphate intermediate. Hence it plays a key role in glycolysis and gluconeogenesis.

Physical form

Lyophilized powder containing Tris HCl, EDTA, magnesium acetate and carbohydrate

Other Notes

One unit will convert 1.0 μmole of α-D-glucose 1-phosphate to α-D-glucose 6-phosphate per min at pH 7.4 at 30 °C.


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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