SRE0116
Salt Active Nuclease (SAN)
recombinant, expressed in Pichia pastoris, Triton-free
Synonym(s):
endonuclease, recombinant nuclease, salt active endonuclease, salt tolerant nuclease
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About This Item
NACRES:
NA.77
UNSPSC Code:
12352204
Recombinant:
expressed in Pichia pastoris
recombinant
expressed in Pichia pastoris
composition
Triton-free
packaging
pkg of 25 kU
shipped in
dry ice
storage temp.
−20°C
Quality Level
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General description
This Salt Active Nuclease (SAN) has been developed to offer the optimal solution for removal of nucleic acids at the near-physiological conditions used in many bioprocessing and biomanufacturing workflows. This novel, nonspecific endonuclease is active over a broad pH range and displays optimum activity at salt concentrations around 175 mM. It is recombinantly expressed in Pichia pastoris.
Application
- Can be used directly in cell medium or harvested supernatant without buffer adjustments
- Ideal for DNA removal directly in mammalian cell media
Features and Benefits
- Active at physiological conditions
- Endotoxin tested Animal origin free without buffer adjustments.
Preparation Note
In solution
Other Notes
- Temperature: 25 – 50 °C
- Salt concentration (NaCl): 50 – 400 mM, optimal: 125 – 250 mM
- Mg2+: > 0.5 mM is required for activity, optimal: 4 - 15 mM
- pH: 6.5 – 9.5, optimal: 7.2 - 8.7
- Not recommended to use in presence of EDTA or urea.
One unit is defined as the amount of enzyme that causes a ΔA260 = 1.0 in 30 minutes at 37 °C in 25 mM Tris-HCl pH 7.6 (@25 °C), 2.5 mM MgCl2, 150 mM NaCl, and 50 μg/ml calf thymus DNA. 25 mM Tris-HCl pH 7.6 (@25 °C), 2.5 mM MgCl2, 150 mM NaCl, and 50 μg/ml calf thymus DNA
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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