SRE0116
Salt Active Nuclease (SAN)
recombinant, expressed in Pichia pastoris, Triton-free
别名:
endonuclease, recombinant nuclease, salt active endonuclease, salt tolerant nuclease
General description
This Salt Active Nuclease (SAN) has been developed to offer the optimal solution for removal of nucleic acids at the near-physiological conditions used in many bioprocessing and biomanufacturing workflows. This novel, nonspecific endonuclease is active over a broad pH range and displays optimum activity at salt concentrations around 175 mM. It is recombinantly expressed in Pichia pastoris.
Application
- Can be used directly in cell medium or harvested supernatant without buffer adjustments
- Ideal for DNA removal directly in mammalian cell media
Features and Benefits
- Active at physiological conditions
- Endotoxin tested Animal origin free without buffer adjustments.
Preparation Note
In solution
Other Notes
- Temperature: 25 – 50 °C
- Salt concentration (NaCl): 50 – 400 mM, optimal: 125 – 250 mM
- Mg2+: > 0.5 mM is required for activity, optimal: 4 - 15 mM
- pH: 6.5 – 9.5, optimal: 7.2 - 8.7
- Not recommended to use in presence of EDTA or urea.
One unit is defined as the amount of enzyme that causes a ΔA260 = 1.0 in 30 minutes at 37 °C in 25 mM Tris-HCl pH 7.6 (@25 °C), 2.5 mM MgCl2, 150 mM NaCl, and 50 μg/ml calf thymus DNA. 25 mM Tris-HCl pH 7.6 (@25 °C), 2.5 mM MgCl2, 150 mM NaCl, and 50 μg/ml calf thymus DNA
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
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