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SRP0100

Sigma-Aldrich

HDAC-1 human

recombinant, expressed in baculovirus infected insect cells, ≥50% (SDS-PAGE)

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Synonym(s):
HD1, Histone deacetylase 1
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected insect cells

Assay

≥50% (SDS-PAGE)

form

aqueous solution

mol wt

56 kDa

packaging

pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

NM_004964

UniProt accession no.

Q13547

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... HDAC1(3065)

General description

Human HDAC1, GenBank Accession No. NM_004964, full length with C-terminal HIS-DDDDK tag (FLAG) and C-terminal His-tag, MW = 56 kDa, expressed in baculovirus expression system.

Biochem/physiol Actions

Useful for the study of enzyme kinetics, screening inhibitors.

Unit Definition

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 130 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide and 10% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Laura De Clerck et al.
Molecular omics, 17(6), 929-938 (2021-09-16)
Histone-based chromatin organization paved the way for eukaryotic genome complexity. Because of their key role in information management, the histone posttranslational modifications (hPTM), which mediate their function, have evolved into an alphabet that has more letters than there are amino

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