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Merck
CN

SRP0169

JMJD2B Active human

recombinant, expressed in baculovirus infected insect cells, ≥40% (SDS-PAGE)

别名:

JHDM3B, JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B, KIAA0876, Lysine-specific demethylase 4B (KDM4B)

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关于此项目

NACRES:
NA.32
UNSPSC Code:
12352202
Form:
aqueous solution
Assay:
≥40% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in baculovirus infected insect cells
Mol wt:
57 kDa
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biological source

human

recombinant

expressed in baculovirus infected insect cells

assay

≥40% (SDS-PAGE)

form

aqueous solution

mol wt

57 kDa

packaging

pkg of 100 μg

concentration

>0.02 mg/mL

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... KDM4B(23030)

General description

Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No.NM_015015) (amino acids 1-500) with C-terminal FLAG tag, MW = 57 kDa, expressed in Sf9 cells using a Baculovirus expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Other Notes

One unit is defined as the amount of enzyme required to demethylate 1 pmol of substrate/min at 37°C.


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