SRP0206
MEG-2 Active human
recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
Synonym(s):
PTPMEG2, PTPN9, Protein tyrosine phosphatase, non-receptor type 9
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
Product Name
MEG-2 Active human, recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
biological source
human
recombinant
expressed in E. coli
assay
≥70% (SDS-PAGE)
form
aqueous solution
mol wt
60.3 kDa
packaging
pkg of 20 μg
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PTPN9(5780)
Application
Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.
General description
Protein tyrosine phosphatase, Catalytic domain, (amino acids 285-593) (Gene bank accession number M83738) with N-terminal GST tag, MW = 60.3 kDa, expressed in an E. coli expression system.
Other Notes
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.01% KCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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