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SRP0219

Sigma-Aldrich

CD45 Active human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

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Synonym(s):
CD45R, Leukocyte common antigen (LCA), PTPRC, Protein tyrosine phosphatase, receptor type, C, T200 leukocyte common antigen
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected insect cells

Assay

≥90% (SDS-PAGE)

form

aqueous solution

mol wt

75 kDa

packaging

pkg of 20 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

NM_080922

UniProt accession no.

P08575

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PTPRC(5788)

General description

Protein tyrosine phosphatase, receptor type C (PTPRC) or leucocyte common antigen CD45 is expressed on T-cells and is found in multiple isoforms. The extracellular domain of the protein is predicted to have an amino-terminal mucin-like segment, a cysteine-rich domain and three fibronectin type 3 domains. The gene encoding this protein is localized on human chromosome 1q31.3.
Protein tyrosine phosphatase. Catalytic domain, (584-1256) (Gene bank accession number NM_080922) with N-terminal His tag, MW = 75 kDa, expressed in a Baculovirus Sf9 expression system.

Application

Useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.

Biochem/physiol Actions

Protein tyrosine phosphatase, receptor type C (PTPRC) or leucocyte common antigen CD45 acts as a protein tyrosine phosphatase in leucocyte signaling. It has a role in flow cytometric diagnostics in leukemia. By dephosphorylation of Src kinases, the protein acts as a regulator of antigen receptor signaling in T and B cells. As a negative regulator of Janus kinases, CD45 inhibits cytokine receptor signaling.

Unit Definition

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Physical form

Formulation: recombinant proteins were eluted in 25 mM Tris-HCL, 75 mM NaCl, pH 8.0, 0.05% Tween, 5 mM DTT and 50% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Alicja Kuban-Jankowska et al.
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Darren Plant
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Neoplasia, 17(11), 839-848 (2015)
Initiation of T cell signaling by CD45 segregation at `close-contacts?
Veronica T Chang
Nature Immunology, 17(5), 574-582 (2016)
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The combination of anti-VEGF blockade (bevacizumab) with immune checkpoint anti-CTLA-4 blockade (ipilimumab) in a phase I study showed tumor endothelial activation and immune cell infiltration that were associated with favorable clinical outcomes in patients with metastatic melanoma. To identify potential
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