SRP0226
NEDD4 Active human
recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)
Synonym(s):
Cell proliferation-inducing gene 53 protein, KIAA0093, PIG53, RPF1, neural precursor cell expressed, developmentally down-regulated 4
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
biological source
human
recombinant
expressed in baculovirus infected insect cells
Assay
≥60% (SDS-PAGE)
form
aqueous solution
mol wt
86 kDa
packaging
pkg of 20 μg
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... NEDD4(4734)
General description
Neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4) has an amino terminal domain, four tryptophan-rich WW domains and a carboxy-terminal homologous to the E6-AP carboxyl terminus (HECT) ubiquitin ligase domain. The gene encoding this protein is localized on human chromosome 15q21.3. Human Nedd4 (GenBank Accession No. NM_006154), amino acids 150-end, with N-terminal DDDDK tag (FLAG) MW = 86kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Application
Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions
Neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4) associates with the PPxY (PY) motifs of the subunits of the human epithelial Na+ channel (hENaC). It is a positive and negative modulator of signaling receptors. The protein is overexpressed in endometrial cancer.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit is defined as the amount of enzyme required to transfer 1 pmol of ubiquitin to substrate/min at 37°C.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Yu-Ru Lee et al.
Science (New York, N.Y.), 364(6441) (2019-05-18)
Activation of tumor suppressors for the treatment of human cancer has been a long sought, yet elusive, strategy. PTEN is a critical tumor suppressive phosphatase that is active in its dimer configuration at the plasma membrane. Polyubiquitination by the ubiquitin
NEDD4 ubiquitin ligase is a putative oncogene in endometrial cancer that activates IGF-1R/PI3K/Akt signaling.
Zhang Y
Gynecologic Oncology, 139(1), 127-133 (2015)
The Nedd4-1 WW Domain Recognizes the PY Motif Peptide through Coupled Folding and Binding Equilibria.
Panwalkar V
Biochemistry, 55(4), 659-674 (2016)
Oncogenic role of the E3 ubiquitin ligase NEDD4-1, a PTEN negative regulator, in non-small-cell lung carcinomas.
Amodio N
The American Journal of Pathology, 177(5), 2622-2634 (2010)
Nicholas M Chesarino et al.
PLoS pathogens, 11(8), e1005095-e1005095 (2015-08-12)
Interferon (IFN)-induced transmembrane protein 3 (IFITM3) is a cell-intrinsic factor that limits influenza virus infections. We previously showed that IFITM3 degradation is increased by its ubiquitination, though the ubiquitin ligase responsible for this modification remained elusive. Here, we demonstrate that
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