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SRP0236

PI3 kinase (p110d/p85a) Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

Synonym(s):

PI3-kinase p110 subunit delta, PIK3CD, Phosphoinositide-3-kinase, catalytic, delta, ptdIns-3-kinase subunit p110-δ

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352200
Specific activity:
≥1.1 pmol/min-μg
Assay:
≥80% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in baculovirus infected insect cells
Concentration:
>0.02 mg/mL

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Product Name

PI3 kinase (p110d/p85a) Active human, recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

biological source

human

recombinant

expressed in baculovirus infected insect cells

assay

≥80% (SDS-PAGE)

form

aqueous solution

specific activity

≥1.1 pmol/min-μg

mol wt

146 kDa (p110δ)
 83.5 kDa (p85α)

packaging

pkg of 20 μg

concentration

>0.02 mg/mL

NCBI accession no.

NM_005026 (p110delta)
XM_043865 (p85alpha)

UniProt accession no.

O00329

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PIK3CD(5293)

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

General description

Complex of N-terminal GST tagged recombinant full-length human p110δ (GenBank Accession No. NM_005026), and recombinant full length, human p85α, GenBank Accession No. XM_043865). Coexpressed in a Baculovirus infected Sf9 cell expression system.

Other Notes

One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 1

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
4062890802
4062860427
40628100728
4062800728

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