SRP0249
PLK2 Active human
recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)
Synonym(s):
Polo-like kinase 2, SNK, Serum-inducible kinase
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
Product Name
PLK2 Active human, recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)
biological source
human
recombinant
expressed in baculovirus infected insect cells
assay
≥60% (SDS-PAGE)
form
aqueous solution
specific activity
≥3.6 pmol/min-μg
mol wt
calculated mol wt 107 kDa
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PLK2(10769)
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
General description
Human Plk2 (GenBank Accession No. NM_006622), full length, with N-terminal GST tag, expressed in a baculovirus infected Sf9 cell expression system. The theoretical MW is 107 kDa.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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M V Ramana Reddy et al.
Bioorganic & medicinal chemistry, 24(4), 521-544 (2016-01-15)
Several families of protein kinases have been shown to play a critical role in the regulation of cell cycle progression, particularly progression through mitosis. These kinase families include the Aurora kinases, the Mps1 gene product and the Polo Like family
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