SRP0256
RB Active human
recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Synonym(s):
OSRC, RB1, Retinoblastoma 1, Retinoblastoma-associated protein, p105-Rb
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
Form:
aqueous solution
Assay:
≥80% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli
Mol wt:
43.5 kDa
biological source
human
recombinant
expressed in E. coli
assay
≥80% (SDS-PAGE)
form
aqueous solution
mol wt
43.5 kDa
packaging
pkg of 200 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... RB1(5925)
General description
The gene encoding Retinoblastoma-associated protein (RB1) is a tumor suppressor gene localized on human chromosome 13q14.2. Human recombinant RB protein (773-end) with N-terminal GST tag (GenBank Accession No. NM_000321), expressed in a E. coli expression system.
Application
Retinoblastoma-associated protein (RB1) has been used in cell activity assay. It is also used as a kinase substrate for the kinase assay and in Western blotting.
Biochem/physiol Actions
Retinoblastoma-associated protein (RB1) modulates the cell cycle. It acts as a repressor of transcription mediated by E2 factor (E2F) and influences transcriptional silencing. RB1 modulates mitochondrial activities and has a role in the organisation of heterochromatin. Mutations in the gene encoding this protein have been associated with childhood retinoblastoma.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.